Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
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United States of America Patent
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Apr 6, 1993
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N/A
app pub date -
Apr 25, 1988
filing date -
Aug 5, 1983
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Expired
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Abstract
A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution.
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