Homogeneous assay system using the nuclease activity of a nucleic acid polymerase

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United States of America Patent

PATENT NO 5210015
SERIAL NO

07563758

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Abstract

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The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.

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Patent Owner(s)

Patent OwnerAddress
ROCHE MOLECULAR SYSTEMS INC4300 HACIENDA DRIVE PLEASANTON CA 94588

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gelfand, David H Oakland, CA 57 7373
Holland, Pamela M Oakland, CA 28 1626
Saiki, Randall K Richmond, CA 23 12130
Watson, Robert M Berkeley, CA 20 2081

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