Quantitation of nucleic acids using the polymerase chain reaction
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United States of America Patent
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Jun 15, 1993
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N/A
app pub date -
Sep 28, 1989
filing date -
Aug 21, 1989
priority date (Note) -
Expired
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Abstract
The present invention provides a method for determining the amount of a target acid segment in a sample by polymerase chain reaction. The method involves the simultaneous amplification of the target nucleic acid segment and an internal standard nucleic acid segment. The amount of amplified DNA from each segment is determined and compared to standard curves to determine the amount of the target nucleic acid segment present in the sample prior to amplification. The method is especially preferred for determining the quantity of a specific mRNA species in a biological sample. Additionally, an internal standard is provided useful for quantitation of multiple mRNA species.
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