Cell-free and whole cell ice nucleators and process for their production
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United States of America Patent
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Jun 29, 1993
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app pub date -
Feb 21, 1991
filing date -
Feb 21, 1991
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Abstract
Microbially produced ice nucleator mixtures which include either cell-free ice nucleator particle mixtures and/or whole cell ice nucleator mixtures. These mixtures are produced in methods which comprises culturing a selected microorganism in a two step process at a first temperature in a first step and at a lower temperature in a second step. The mciroorganisms include Erwinia, Pseudomonas and Escherichia coil. These methods produce ice nucleator mixtures having increased concentrations of ice nucleating sites per given weight or volume of ice nucleator material.
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