Enzyme immobilzation on a macropermable agglomerate of microporous .alpha.-a
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United States of America Patent
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Jul 20, 1993
Grant Date -
N/A
app pub date -
Jul 30, 1991
filing date -
Aug 2, 1990
priority date (Note) -
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Abstract
Enzymes are immboilized on an inorganic carrier which is a round, macropermeable agglomerate having a mulberry-type structure formed from spherical microporous .alpha.-aluminum oxide hydroxide particles. An organic polycondensate having free organic functional groups as enzyme binding sites penetrates the agglomerate. The .alpha.-aluminum oxide hydroxide particles of the agglomerate retain essentially their form prior to agglomeration. The polycondensate is prepared by reacting a polyimine with a dialdehyde or an epoxy compound. Preferably, the polyimine is polyethyleneimine, the dialdehyde is glutardialdehyde and the epoxy compound is an epoxy-functional silicon resin, an aliphatic diepoxide or an aliphatic triepoxide. The agglomerate is prepared by granulating the .alpha.-aluminum oxide hydroxide particles in the presence of the polyimine to form the agglomerate having the polyimine dispersed throughout and then reacting the polyimine with the dialdehyde or the epoxy compound. A preferred enzyme is glucose isomerase.
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