Reagent for use in automatic analyzers for distinguisher leukocyte sub-populations in blood samples
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United States of America Patent
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Aug 3, 1993
Grant Date -
N/A
app pub date -
Nov 19, 1990
filing date -
Nov 20, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention relates to a reagent and to a method of using the same for automatically determining at least one leukocyte sub-population from a total blood sample. Using this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.
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