Antigen retrieval in formalin-fixed tissues using microwave energy
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United States of America Patent
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Sep 14, 1993
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Jan 31, 1991
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Jan 31, 1991
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Abstract
A method of immunologically staining a formalin-fixed tissue preparation, which comprises (a) subjecting a formalin-fixed tissue preparation to microwave energy while the tissue preparation is submersed in water for a time sufficient to increase immunostaining efficiency; (b) removing the tissue preparation from the water and cooling; and (c) contacting the tissue preparation with an immunological staining reagent.
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