Carrageeman-immobilized esterase
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United States of America Patent
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Nov 16, 1993
Grant Date -
N/A
app pub date -
Jun 14, 1991
filing date -
Jun 14, 1991
priority date (Note) -
Expired
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Abstract
Carrageenan-immobilized enzymes are prepared that are stable and retain high activity toward substrates when used in substantially water-immiscible organic solvents. The carrageenan-immobilized enzymes are preferably prepared by introducing droplets of an enzyme/carrageenan solution into a chilled alcohol saturated with a potassium salt and hardening the droplets in the alcohol. The alcohol is selected from n-butanol, benzyl alcohol, crotyl alcohol, n-propanol, isopropanol and sec-butanol. Reactions catalyzed by a .kappa.-carrageenan-immobilized esterase include steroselective transesterifications and hydrolysis reactions.
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