Immunoassay for pyridostigmine
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United States of America Patent
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Dec 7, 1993
Grant Date -
N/A
app pub date -
Apr 10, 1989
filing date -
Apr 11, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A radioimmunoassay for pyridostigmine in biological fluids. The method disclosed can detect 250 pg of pyridostigmine per ml of biological fluid using 0.1 ml of biological fluid. The antibody used in this method is raised from an antigen produced by diazotizing para-aminobenzoic acid and reacting the diazotized material with pyridostigmine to form a pyridostigmine-hapten solution, which is then reacted with bovine serum albumen. The antibody used in the disclosed method has a cross-reactivity with the major metabolites of pyridostigmine of less than 2%.
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