Process for the isolation and purification of taxol and taxanes from Taxus spp
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United States of America Patent
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Jan 18, 1994
Grant Date -
N/A
app pub date -
Dec 7, 1992
filing date -
Dec 7, 1992
priority date (Note) -
Expired
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Abstract
A process for the separation of taxanes, particularly taxol, from ornamental yew tissue is described. The process involves a specific solvent mixture of ethanol and water which is between 50 to 80 percent by volume ethanol and decolorizing of the resulting extract using activated carbon containing the crude taxanes. The taxanes are separated from the crude extract by a normal phase chromatographic step which preferably is through vacuum or medium pressure column chromatographic separation, using inexpensive silica gel as an absorbent.
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