Capture sandwich hybridization method and composition
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United States of America Patent
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Feb 22, 1994
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N/A
app pub date -
Oct 30, 1992
filing date -
Apr 27, 1984
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Abstract
This invention provides methods and compositions for the detection of a target genetic material having a desired base sequence or gene. Also disclosed are methods and compositions for the detection of mutants. The methods and compositions are based in part upon techniques which utilize two labeled single-stranded polynucleotide segments which are complementary to different portions of the genetic material. The methods and compositions of this invention result in the formation of a double hybrid and/or multihybrid, and can furthermore be employed in an attached system, i.e., a matrix-bound entity capable of binding to a polynucleotide probe entity.
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