Process for producing foreign protein in Escherichia coli
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United States of America Patent
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Apr 19, 1994
Grant Date -
N/A
app pub date -
Feb 8, 1991
filing date -
Feb 9, 1990
priority date (Note) -
Expired
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Abstract
A process of producing a foreign protein is provided which comprises transforming E. coli with a plasmid carrying a fused DNA having a DNA fragment encoding a foreign protein located downstream of a transcription initiation signal and translation initiation signal both derived from an appropriate T4 phage gene, infecting the transformant with a T4 phase denB and/or alc mutant at a low multiplicity of infection, and then culturing the infected transformant. The desired foreign protein can be produced in an extremely high yield, as compared to the prior art technique.
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