Method for the purification of Erwinia L-asparaginase
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United States of America Patent
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May 10, 1994
Grant Date -
N/A
app pub date -
Jun 25, 1993
filing date -
Aug 2, 1990
priority date (Note) -
Expired
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Abstract
A process is provided for the purification of L-asparaginase by contacting a crude extract of L-asparaginase with a solid medium having cation exchange groups so as to adsorb L-asparaginase on the support and eluting adsorbed L-asparaginase from the support. The cation exchange groups comprise sulphonate groups and the elution step is carried out at a pH which is higher than the pH used in the contacting step.
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