Method and apparatus for eluting proteins in affinity membrane process
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United States of America Patent
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May 10, 1994
Issued Date -
N/A
app pub date -
Mar 23, 1993
filing date -
Oct 31, 1988
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Expired
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Abstract
This invention relates to methods and apparatus for carrying out affinity separations of proteins under conditions optimized for recovering biologically active protein. The method utilizes a membrane-bound ligand first to capture and separate a ligate from a fluid mixture and subsequently to release said ligate in purified form. The present methods enhance the yield of active ligate recovered in the process. The invention has particular relevance to the recovery and purification of proteins from mixtures.
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