Method of setting up a flow cytometer
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United States of America Patent
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May 24, 1994
Issued Date -
N/A
app pub date -
Oct 27, 1992
filing date -
Dec 24, 1984
priority date (Note) -
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Abstract
A method of setting up a flow cytometer using microbeads fluorescing at a plurality of wavelengths, control cells for each of said wavelengths, certified blank microbeads. The blank microbeads are run and the PMT voltages are adjusted so that the blank microbeads are on scale on the flow cytometer. The control cells are run and the photomultiplier tube voltages are adjusted so that the single population falls in selected channels. The fluorescent microbeads are run on the flow cytometer and the Target Channels for the flow cytometer are determined.
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