Importance

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Abstract

A method for isolating tissue repair promoting substances from human or animal blood, which method comprises collecting the human or animal blood from a single human or animal individual in a first container of a container system comprising at least first and second interconnected containers; centrifuging the container system containing said blood so as to separate the blood in various fractions including a plasma fraction; transferring at least part of the plasma fraction to said second container of the container system; subjecting the plasma fraction in said second container to a low temperature so as to obtain a precipitate comprising tissue repair promoting substances; concentrating said precipitate in said second container so as to obtain a first fraction comprising a major part of the non-precipitated material, and a second fraction comprising at least the major part of the precipitate and a minor part of the non-precipitated material; and separating said second fraction comprising the tissue repair promoting substances from said first fraction within the second container; and optionally heating said second fraction within one of the containers of the container system so as to dissolve the isolated precipitated tissue repair promoting substances. Furthermore, fractions comprising tissue repair promoting substances derived from blood cells may be isolated within the container system, said fractions optionally being combined with the second fraction comprising tissue repair promoting substances derived from blood plasma. At least part of the fractions obtained may be kept for application to a tissue of the human or animal individual from whom the blood was collected.

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First Claim