Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
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United States of America Patent
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Jun 21, 1994
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N/A
app pub date -
Dec 22, 1989
filing date -
Dec 22, 1989
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Abstract
Methods are provided for the replication and amplification of RNA sequences by thermostable DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 92 kDa Taq, 64 kDa Taq, and Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
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