Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
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United States of America Patent
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Aug 2, 1994
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N/A
app pub date -
Feb 22, 1993
filing date -
Feb 25, 1986
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Abstract
There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.
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