Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
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United States of America Patent
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Aug 2, 1994
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app pub date -
Jun 28, 1993
filing date -
Jan 15, 1992
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Abstract
Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposited on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. The biodetectors may be employed for mediatorless detection of a wide variety of analytyes depending on the oxidase employed.
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