Method for detecting intestinal pathogen dientamoeba fragilis

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United States of America Patent

PATENT NO 5334509
SERIAL NO

07964874

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Abstract

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A method and apparatus for producing detectable intestinal parasites. The method includes obtaining an intestinal mucosa sample (e.g. feces) having intestinal parasites, such as Dienamoeba fragilis; and contacting the obtained intestinal mucosa sample with an acridine base compound (e.g. acridine orange and/or acridine yellow, etc.) such that the intestinal parasites become differentially stained and detectable by a human eye when viewed through a fluorescence microscope. The apparatus includes a kit or the like which includes at least one vessel or vial. Preferably, two vials are contained within the kit with one vial having an isotonic salt solution including a salt, such as sodium chloride, potassium phosphate, etc., and the other vial containing an acridine biological staining compound.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Riordan, Neil H 7715 E. 32nd North, Wichita, KS 67226 35 187

Cited Art Landscape

Patent Info (Count) # Cites Year
 
Technicon Instruments Corporation (1)
* 5075556 Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood 19 1989
 
Other [Check patent profile for assignment information] (1)
* 4906561 Nuclear isolation medium and procedure for separating cell nuclei 10 1987
 
SKB AND ZYNAXIS CELL SCIENCE, INC. A CORP. OF PENNSYLVANIA (1)
* 4783401 Viable cell labelling 55 1986
 
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V. (1)
* 4751188 Method for the simultaneous quantitative determination of cells and reagent therefor 47 1983
* Cited By Examiner

Patent Citation Ranking

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Patent Info (Count) # Cites Year
 
Other [Check patent profile for assignment information] (2)
8372599 Culture medium and a method for detection of parasites 1 2004
9365818 Culture medium and a method for detection of parasites 0 2012
 
OLYMPUS CORPORATION (1)
* 2011/0236,895 METHOD FOR PREPARING SAMPLE, SOLUTION FOR PREPARING SAMPLE AND STOOL COLLECTION KIT METHOD FOR ANALYZING A NUCLEIC ACID 0 2011
 
MEDICAL CHEMICAL CORPORATION (1)
8338130 Universal fecal fixative comprising a low molecular weight alcohol, a zinc salt and an organic acid 0 2011
 
CHEMIMAGE CORPORATION (4)
7057721 Wide field method for detecting pathogenic microorganisms 24 2003
* 2005/0185,178 WIDE FIELD METHOD FOR DETECTING PATHOGENIC MICROORGANISMS 6 2003
8395769 Method for analysis of pathogenic microorganisms using raman spectroscopic techniques 5 2010
* 2011/0007,309 Method for Analysis of Pathogenic Microorganisms Using Raman Spectroscopic Techniques 3 2010
* Cited By Examiner