Methods for determining anti-phospholipid and anti-cardiolipin antibodies
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United States of America Patent
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Sep 6, 1994
Grant Date -
N/A
app pub date -
Dec 6, 1991
filing date -
Apr 6, 1990
priority date (Note) -
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Abstract
A method for determining whether antiphospholipid antibodies are present in a sample is disclosed. The method comprises contacting the sample with a negatively charged phospholipid and with .beta.-2-glycoprotein-I or a homolog or analog thereof and determining whether any antiphospholipid antibodies have bound to the contacted phospholipid and .beta.-2-glycoprotein-I, wherein detection of binding of antiphospholipid antibodies to the phospholipid and .beta.-2-glycoprotein-I, is indicative that antiphospholipid antibodies are present in the sample. Also disclosed is a method for determining whether a negatively charged phospholipid is present in a sample, a kit for use in an assay for determining whether antiphospholipid antibodies are present in a sample, a kit for use in an assay for determining whether a negatively charged phospholipid is present in a sample, a method for determining whether antiphospholipid antibodies in a sample are antibodies to an autoimmune disease or antibodies to an infectious disease and a method for determining whether antiphospholipid antibodies to an autoimmune disease and antiphospholipid antibodies to an infectious disease are present in a sample.
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