Inosinic acid dehydrogenase assay

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United States of America Patent

PATENT NO 5358855
SERIAL NO

07882647

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Abstract

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A cyto(histo)chemical technique for determining inosine 5'-monophosphate dehydrogenase (IMPD) presence and concentration within one or more morphologically classified or classifiable cells taken from an animal or a human subject. Cell monolayers are prepared, fixed and stained according to a specified procedure in which inosine 5'-monophosphate (as enzyme substrate), nicotinamide adenine dinucleotide (NAD), phenazine methosulfate (PMS) and nitro blue tetrazolium (NBT) as a diazonium salt together produce a granular, dark blue deposit of formazan generally proportional to the IMPD activity in the cell. Cells are evaluated either visually or by image analysis, with a computerized image analyzer or other imaging system, according to accepted biostatistical methods and standards.

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Patent Owner(s)

Patent OwnerAddress
MEDICAL COLLEGE OF PENNSYLVANIA THE330 HENRY AVENUE PHILADELPHIA PA 19129

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Markovic, Nenad S Novi Sad, YU 3 12
Markovic, Olivera T Philadelphia, PA 2 7
Markovic, Svetomir N Philadelphia, PA 96 3384
Puglia, Charles D Glenside, PA 1 0
Roberts, Jay Rosemont, PA 9 19

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