Method of preparing human conjunctival mast cells for mast cell stabilization assays
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United States of America Patent
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Nov 1, 1994
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N/A
app pub date -
Oct 8, 1993
filing date -
Oct 8, 1993
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Abstract
By allowing the mast cell enriched population of human conjunctival tissue cells to incubate for a minimum of about forty (40) hours post enzymatic digestion, the treatment window between spontaneous histamine release and anti-human IgE stimulated histamine release is increased. Culturing the human conjunctival tissue mast cells decreases the spontaneous release of histamine and increases the anti-IgE stimulated histamine release, greater than ten fold over spontaneous, at time points over forty (40) hours. This treatment window is sufficient to detect a compound's stabilizing or anti-allergic activity.
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