Methods and containment system for storing, reconstituting, dispensing and harvesting cell culture media
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United States of America Patent
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Nov 8, 1994
Grant Date -
N/A
app pub date -
Feb 10, 1993
filing date -
Feb 10, 1993
priority date (Note) -
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Abstract
A cell culture media containment system is provided having a mixing bag substantially enclosed within a storage bag. Powdered cell culture media and other constituents are introduced into the mixing bag and are mixed therein. Thereafter, the reconstituted media is conveyed from the mixing bag into the storage bag undergoing sterilization during conveyance therebetween. The reconstituted cell culture media may then be stored in a sterilized condition in the storage bag or maybe dispensed into a cell growth container. In another embodiment of the present invention a plurality of storage bags or mixing bags may be utilized so that reconstituted cell culture media may be both mixed and dispensed while protein-laden cell culture media may be simultaneously harvested back into another of the plurality of storage bags.
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