Oligonucleotides with selectably cleavable and/or abasic sites
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United States of America Patent
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Nov 22, 1994
Issued Date -
N/A
app pub date -
Jul 24, 1991
filing date -
Oct 16, 1984
priority date (Note) -
Expired
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Importance
Abstract
A modified polynucleotide containing at least one cleavable or abasic site as shown below. ##STR1## DNA.sub.1 is a first segment of DNA; DNA.sub.2 is a second segment of DNA; and R.sub.m is C.sub.1 to C.sub.16 alkylene or an oxytheylene oligomer --(CH.sub.2 CH.sub.2 O).sub.z -- where z is an interger in the range of 1 to 16 inclusive, and R.sub.n is selected from the group consisting of ##STR2## Such polynucleotides are useful in solid phase hybridizations because they permit the release of a label from the solid support after the hybridization reaction.
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