Repetitive DNA sequence specific for mycobacterium tuberculosis to be used for the diagnosis of tuberculosis
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United States of America Patent
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Dec 6, 1994
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app pub date -
Oct 1, 1991
filing date -
Sep 28, 1990
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Abstract
A novel composition and/or methods for the diagnosis of tuberculosis wherein the composition comprises a repetitive DNA segment that is specific for members of the Mycobacterium tuberculosis complex. The DNA segment repeats in the chromosome of Mycobacterium tuberculosis complex, and is conserved in all copies of the chromosomes. A method comprises using an entire repetitive DNA sequence, or any part thereof, as a hybridization probe for the direct detection of Mycobacterium tuberculosis complex in clinical material. In another method, a smaller portion of an entire repetitive DNA sequence is amplified using polymerase chain reaction, yielding a 123 base-pair product.
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