D-pantolactone hydrolase and process for the preparation thereof
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United States of America Patent
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Dec 13, 1994
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N/A
app pub date -
Jun 2, 1992
filing date -
Oct 5, 1990
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Abstract
The invention is drawn to a new enzyme that selectively hydrolyzes D-pantolactone in D,L-pantolactone and has the following characteristics: (a) action: acts on pantolactone to produce the corresponding acid; (b) specificity for substrate: acts specifically on D-pantolactone but not on L-pantolactone; (c) pH stability: stable at pH5-9; (d) optimal pH: 7.0-7.5; (e) optimal temperature: ca. 50.degree. C.; and (f) effect of various metal ions or inhibitors: inhibited by Cd.sup.2+, Hg.sup.2+ or Cu.sup.2+ EDTA, as well as a process for the preparation thereof. The enzyme is preferably produced by cultivating the microorganisms of the genera Fusarium, Cylindrocarpon, and Gibberella, and most preferably the microorganism Fusarium oxysporum IFO 5942.
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