Expression of interferon genes in Chinese hamster ovary cells
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United States of America Patent
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Dec 27, 1994
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app pub date -
Jan 9, 1992
filing date -
Nov 1, 1982
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Abstract
DNA constructs are prepared which operably link human interferon genes, selective, eukaryotic marker genes, and promoter and expression control sequences for the expression of human interferon in Chinese hamster ovary (CHO) cells or progeny thereof. The human recombinant interferon so produced contains glycans which are a subset of the population of glycans which are contained in the native counterpart, and may be used in therapeutic formulations. The CHO cells yield high levels of human interferon with no detectable amounts of host, IFN, either constitutive or inductive.
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