Supercritical fluid disruption of and extraction from microbial cells
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United States of America Patent
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Jan 10, 1995
Issued Date -
N/A
app pub date -
Sep 29, 1992
filing date -
Jul 20, 1989
priority date (Note) -
Expired
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Abstract
The invention involves the supercritical or near-critical fluid disruption of microbial cells and extraction of intracellular components. First, a solvent that is a gas at ambient conditions and that has a critical temperature of between 0.degree. and 100.degree. C. is selected. This solvent is brought to near-critical pressure or higher and to near-critical temperature. The solvent then is combined with a slurry of cells to saturate the cells with the solvent under the prescribed conditions. Next, the pressure is released to cause a pressure drop which results in partial disruption of the cell membrane and release of solvent and other materials from the cell. Novel apparatus and associated methods are provided for carrying out the foregoing process continuously.
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