Method for the isolation and purification of taxane derivatives
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United States of America Patent
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Jan 10, 1995
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app pub date -
Jul 16, 1992
filing date -
Nov 2, 1990
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Abstract
An improved method for isolating certain clinically important taxane derivatives from the crude extract of a naturally occurring Taxus species comprising treating the extract by reverse phase liquid chromatography on an adsorbent; causing the taxane derivatives to be adsorbed on the adsorbent; and recovering the taxane derivatives from the adsorbent by elution with an elutant. The taxane derivatives thus isolated in pure form are taxol, taxol-7-xyloside, 10-deacetyitaxol, 10-deacetyltaxol-7-xyloside, cephalomannine, cephalomannine-7-xyloside, 10-deactylcephalomannine-7-xyloside, baccatin III, 10-deacetylbaccatin III, baccatin VI, and brevitaxane A.
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