Biosensor containing a biochemical substance immobilized on a layer of an olefinic-unsaturated, epoxyfunctional polyether
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United States of America Patent
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Feb 14, 1995
Grant Date -
N/A
app pub date -
Mar 22, 1993
filing date -
Mar 23, 1992
priority date (Note) -
Expired
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Abstract
A biosensor is prepared having a selective detection system containing a biochemical substance such as an enzyme immobilized by reaction with epoxy groups of an olefinic-unsaturated, epoxyfunctional polyether. Prior to immobilization, the polyether is applied to a carrier and crosslinked by treatment with high-energy radiation or peroxide to form a layer. After reacting the biochemical substance with epoxy groups, non-reacted epoxy groups are reacted with a compound containing an amino group and/or a carboxyl group such as an amino acid. Before immobilizing of the biochemical substance and after crosslinking, the polyether may be hydrophilized by reacting some of the epoxy groups with a hydrophilic compound such as an amino acid.
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