Expression of tetanus toxin fragment C in yeast
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United States of America Patent
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Feb 14, 1995
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app pub date -
Nov 27, 1990
filing date -
Nov 28, 1989
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Abstract
Expression of tetanus toxin fragment C is accomplished employing a DNA coding sequence having a (G+C)-content that has been increased in the region from nucleotide 410 to the 3' end of the coding sequence relative to the wild-type DNA sequence. This allows the production of complete mRNA transcripts. Typically the (G+C)-content is increased in the following regions: (i) nucleotides 510-710, (ii) nucleotides 650-850, (iii) nucleotides 800-1100, (iv) nucleotides 900-1200 and (v) nucleotides 1100 to the 3' end of the coding sequence. The (G+C)-content may also be increased in the region of nucleotides 410-610. These regions in the wild-type DNA encompass terminator sequences.
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