Method for detecting a nucleotide at a specific location within a nucleic acid using exonuclease activity
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United States of America Patent
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Feb 21, 1995
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N/A
app pub date -
Dec 3, 1991
filing date -
Mar 21, 1989
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Expired
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Abstract
A novel technique for determining the existence or nonexistence of a test nucleotide on a strand of DNA is provided. The determination advantageously uses an exonucleolytic agent that is capable of retaining a labeled nucleotide in a primer if there is a match between the test nucleotide on the strand of DNA and the complementary nucleotide on the primer, but not if there is a mismatch. The presence or absence of the test nucleotide then may be established by determining whether the label is preserved or lost following the reaction.
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