Biosensor containing a biochemical substance immobilized on a layer of olefinic-unsaturated, epoxy functional cross-linked polysiloxane
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United States of America Patent
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Apr 18, 1995
Grant Date -
N/A
app pub date -
Mar 22, 1993
filing date -
Mar 23, 1992
priority date (Note) -
Expired
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Abstract
A biosensor is prepared having a selective detection system containing a biochemical substance such as an enzyme immobilized on an olefinic-unsaturated, epoxyfunctional polysiloxane. Prior to immobilization of the biochemical substance, the polysiloxane is applied as a layer to a carrier and cross-linked by treatment with high-energy radiation. A biochemical substance is reacted with epoxy groups of the cross-linked polysiloxane. Any non-reacted epoxy groups are reacted with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group to stabilize. After cross-linking and before reacting of the biochemical substance, the cross-linked polysiloxane can be hydrophilized by reacting some of the epoxy groups with a hydrophilic compound containing a reactive group.
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