Method and system for determining bioactive substances
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United States of America Patent
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May 2, 1995
Grant Date -
N/A
app pub date -
Mar 30, 1993
filing date -
Mar 30, 1993
priority date (Note) -
Expired
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Abstract
An apparatus and method for measuring glutamine and glucose in certain cell cultures and body fluids in the presence of interfering endogenous compounds e.g. ascorbic acid, uric acid or glutamic acid. Prior to enzymatic degradation and amperometric detection of the products or elements resulting from the degradation, the interfering substances are retained by an anion exchanger disposed upstream of the enzymatic degradation site. The conditions of the method are controlled to utilize the difference of isoelectric points of the measured biosubstance and of the interfering compounds respectively.
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