Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
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United States of America Patent
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May 23, 1995
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Jan 5, 1993
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Jul 24, 1990
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Abstract
Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bags into the amplification reaction products and treating the products by enzymatic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications.
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