Method for isolating cells from tissue with a composition containing collagenase and chymopapin
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United States of America Patent
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Jun 13, 1995
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N/A
app pub date -
Sep 23, 1994
filing date -
Apr 16, 1993
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Abstract
Proteolytic enzyme compositions and processes for digesting connective tissue are provided. The enzyme compositions include collagenase, which is essentially free of toxins and non-collagen specific components, and chymopapain, which is essentially free of toxins. The enzyme compositions are used for dissociating microvessel cells from connective tissue. Recovered microvessel cells are incorporated into artificial vessel grafts. The composition preferrably contains collagenase having an activity of about 43 nkat/ml to about 51 nkat/ml and chymopapin having an activity of about 0.22 nkat/ml to about 0.44 nkat/ml.
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