Method for separating rare cells from a population of cells
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United States of America Patent
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Jul 11, 1995
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N/A
app pub date -
Jan 31, 1994
filing date -
Jan 31, 1994
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Abstract
A method for isolating and enriching rare cells, including fetal nucleated erythrocytes from a peripheral blood sample is described. The method includes two centrifugation steps, the first a bulk separation step to enrich erythrocytes from other blood components. The second centrifugation comprises a colloidal density gradient medium dispersed in a hypertonic meltable gel. In one aspect of the invention, maternal erythrocytes may be hemolyzed prior to the second density gradient centrifugation to provide additional enrichment for fetal nucleated erythrocytes.
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