Device for maintaining and growing human stem and/or hematopoietics cells
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United States of America Patent
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Oct 17, 1995
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N/A
app pub date -
Jan 6, 1994
filing date -
Jun 15, 1989
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Abstract
Devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The stromal cells and hematopoietic cells are maintained separately and both the adherent and non-adherent cells are harvested.
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