Indirect fluorescent assay of blood samples

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United States of America Patent

PATENT NO 5460979
SERIAL NO

08192629

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labelled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labelled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labelled antibodies will thus not interfere with the analysis.

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Patent Owner(s)

Patent OwnerAddress
QBC DIAGNOSTICS INC200 SHADYLANE DRIVE PHILIPSBURG PA 16866

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Levine, Robert A Guilford, CT 145 3889
Mercolino, Thomas J Stockton, NJ 25 463
Recktenwald, Diether J Cupertino, CA 12 612
Terstappen, Leon W M M Palo Alto, CA 41 2272
Wardlaw, Stephen C Old Saybrook, CT 181 4758

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