Sexing method of bovine embyros
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United States of America Patent
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Oct 24, 1995
Grant Date -
N/A
app pub date -
Dec 2, 1992
filing date -
Dec 13, 1991
priority date (Note) -
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Abstract
The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.
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