US Patent No: 5,468,613

Number of patents in Portfolio can not be more than 2000

Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids

Stats

ATTORNEY / AGENT: (SPONSORED)
 

Importance

Loading Importance Indicators... loading....

Abstract

Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.

Loading the Abstract Image... loading....

First Claim

Related Publications

Loading Related Publications... loading....

Patent Owner(s)

Patent OwnerAddressTotal Patents
ROCHE MOLECULAR SYSTEMS, INC.PLEASANTON, CA285

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Erlich, Henry A Oakland, CA 26 5197
Horn, Glenn Emeryville, CA 6 1234
Mullis, Kary B Newport Beach, CA 35 8149
Saiki, Randall K Richmond, CA 23 5865

Cited Art

Patent Info (Count) # Cites Year
 
HOFFMANN-LA ROCHE INC. (2)
4,582,788 HLA typing method and cDNA probes used therein 146 1983
4,683,194 Method for detection of polymorphic restriction sites and nucleic acid sequences 248 1985
 
ROCHE MOLECULAR SYSTEMS, INC. (2)
4,683,202 Process for amplifying nucleic acid sequences 2766 1985
4,683,195 Process for amplifying, detecting, and/or-cloning nucleic acid sequences 2437 1986
 
UNIVERSITY OF MASSACHUSETTS (2)
4,965,189 Probes for the determination of the proclivity for development of autoimmune diseases 10 1986
5,059,519 Oligonucleotide probes for the determination of the proclivity for development of autoimmune diseases 6 1988
 
VIRGINIA MASON RESEARCH CENTER (2)
5,039,606 Diagnostic probe for diabetes type I predisposition 7 1987
4,971,902 Diagnostic probe for rheumatoid arthritis predisposition 6 1987
 
WAKUNAGA SEIYAKU KABUSHIKI KAISHA (1)
4,605,735 Oligonucleotide derivatives 83 1984

Patent Citation Ranking

Forward Cites

Patent Info (Count) # Cites Year
 
ESOTERIX GENETIC LABORATORIES, LLC (21)
5,670,325 Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample 47 1996
5,928,870 Methods for the detection of loss of heterozygosity 57 1997
6,100,029 Methods for the detection of chromosomal aberrations 51 1997
6,146,828 Methods for detecting differences in RNA expression levels and uses therefor 51 1998
6,020,137 Methods for the detection of loss of heterozygosity 39 1998
6,280,947 Methods for detecting nucleotide insertion or deletion using primer extension 25 1999
6,919,174 Methods for disease detection 5 2000
6,300,077 Methods for the detection of nucleic acids 22 2000
6,964,846 Methods for detecting nucleic acids indicative of cancer 5 2000
6,849,403 Apparatus and method for drug screening 3 2000
6,503,718 Methods for detecting mutations using primer extension for detecting disease 15 2001
6,428,964 Method for alteration detection 20 2001
6,498,012 Methods for detecting mutations using primer extension for detecting disease 11 2001
6,475,738 Methods for detecting mutations using primer extension for detecting disease 41 2001
6,482,595 Methods for detecting mutations using primer extension 13 2001
6,750,020 Method for alteration detection 4 2001
7,776,524 Methods for analysis of molecular events 1 2003
7,981,607 Method for detecting recombinant event 1 2005
7,811,757 Methods for disease detection 0 2006
8,409,829 Methods for analysis of molecular events 0 2010
8,389,220 Method for detecting a recombinant event 0 2011
 
XDX, INC. (9)
7,026,121 Methods and compositions for diagnosing and monitoring transplant rejection 26 2002
7,235,358 Methods and compositions for diagnosing and monitoring transplant rejection 14 2002
7,691,569 Methods and compositions for diagnosing and monitoring transplant rejection 0 2003
7,645,575 Genes useful for diagnosing and monitoring inflammation related disorders 0 2005
7,993,832 Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders 0 2007
7,829,286 Methods and compositions for diagnosing and monitoring transplant rejection 0 2008
7,785,797 Methods and compositions for diagnosing and monitoring transplant rejection 0 2009
7,960,121 Methods and compositions for diagnosing and monitoring transplant rejection 1 2010
7,879,556 Methods and compositions for diagnosing and monitoring transplant rejection 1 2010
 
EXACT SCIENCES CORPORATION (6)
5,741,650 Methods for detecting colon cancer from stool samples 39 1996
5,952,178 Methods for disease diagnosis from stool samples 40 1998
6,268,136 Methods for stool sample preparation 20 1998
6,303,304 Methods for disease diagnosis from stool samples 19 1999
6,551,777 Methods for preserving DNA integrity 16 2000
6,406,857 Methods for stool sample preparation 17 2001
 
LABORATORY CORPORATION OF AMERICA HOLDINGS (6)
6,905,827 Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases 59 2002
7,604,936 Methods and compositions for diagnosis and monitoring auto immune and chronic inflammatory diseases 0 2003
7,579,148 Methods and compositions for diagnosing or monitoring autoimmune and chronic inflammatory diseases 0 2004
8,148,067 Methods for diagnosing and monitoring the status of systemic lupus erythematosus 0 2007
7,771,950 Methods and compositions for diagnosing and monitoring auto immune and chronic inflammatory diseases 0 2009
8,110,364 Methods and compositions for diagnosing or monitoring autoimmune and chronic inflammatory diseases 0 2010
 
INSTITUTE FOR SYSTEMS BIOLOGY (3)
7,300,755 Methods for haplotyping genomic DNA 4 2004
7,615,350 Methods for haplotyping genomic DNA 1 2007
7,972,791 Methods for haplotyping genomic DNA 0 2009
 
BIOMERIEUX (2)
6,818,393 DNA sequences coding for the DR beta-chain locus of the human lymphocyte antigen complex and polypeptides, diagnostic typing processes and products related thereto 1 1995
5,976,789 System of probes enabling HLA-DR typing to be performed, and typing method using said probes 6 1995
 
CLONTECH LABORATORIES, INC. (2)
5,994,076 Methods of assaying differential expression 76 1997
6,489,455 Methods of assaying differential expression 5 1999
 
GEN-PROBE INCORPORATED (2)
6,280,952 Two-step hybridization and capture of a polynucleotide 19 2000
6,534,273 Two-step hybridization and capture of a polynucleotide 21 2001
 
LIFE TECHNOLOGIES CORPORATION (2)
6,306,588 Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof 6 1998
7,501,237 Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof 9 2001
 
MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH (2)
7,368,233 Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm 5 2000
7,981,612 Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm 0 2008
 
AFFYMETRIX, INC. (1)
6,852,488 Identifying a base in a nucleic acid 0 2001
 
BLOOD CENTER RESEARCH FOUNDATION (1)
6,503,707 Method for genetic typing 2 1996
 
CURAGEN CORPORATION (1)
6,355,423 Methods and devices for measuring differential gene expression 12 1998
 
DADE BEHRING MARBURG GMBH (1)
5,882,867 Detection of nucleic acids by formation of template-dependent product 56 1995
 
EXPRESSION DIAGNOSTICS, INC. (1)
7,892,745 Methods and compositions for diagnosing and monitoring transplant rejection 0 2009
 
HANDYLAB, INC. (1)
8,105,477 System and method for electrochemical detection of biological compounds 0 2010
 
INVITROGEN CORPORATION (1)
6,528,256 Methods for identification and isolation of specific nucleotide sequences in cDNA and genomic DNA 1 2000
 
ISIS INNOVATION LIMITED (1)
6,387,615 Methods and materials for the diagnosis or prognosis of asthma 5 1998
 
KABUSHIKI KAISHA DNAFORM (1)
8,206,902 Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same 0 2004
 
KIMBERLY-CLARK WORLDWIDE, INC. (1)
7,179,603 Detection and identification of enteric bacteria 0 2003
 
MASSACHUSETTS INSTITUTE OF TECHNOLOGY (1)
6,703,228 Methods and products related to genotyping and DNA analysis 24 1999
 
RIKEN (1)
7,803,579 Process for amplifying nucleic acid 0 2003
 
ROCHE MOLECULAR SYSTEMS, INC. (1)
5,604,099 Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids 64 1995
 
STICHTING CENTRAAL LABORATORIUM VAN DE BLOEDTRANS-FUSIEDIENST VAN HET (1)
6,379,881 Nucleic acids and methods for the discrimination between syncytium inducing and non syncytium inducing variants of the human immunodeficiency virus 0 1996
 
VYSIS, INC. (1)
RE37891 Target and background capture methods with amplification for affinity assays 35 2000
 
WASHINGTON UNIVERSITY (1)
8,080,371 Markers for addiction 0 2007
 
WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH (1)
6,203,993 Methods for the detection of nucleic acids 60 2000