Article for performing immunological assays utilizing organic dyes to immobilize immunologically reactive components to a solid phase support and methods for producing and utilizing same
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United States of America Patent
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Nov 21, 1995
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N/A
app pub date -
Aug 18, 1993
filing date -
Jul 5, 1988
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Abstract
An article for performing solid-phase immunological assays and methods for performing assays utilizing such article. A solid-phase support suitable for immunological assays is stained with an organic dye that has a net-positive charge and a hydrophobic aromatic ring structure. An immunologically reactive component having a net-negative charge, such as a whole cell, is securely immobolized by the dye to the support through noncovalent interactions. A sample of biological fluid may be added to the article to which the immunologically reactive component has been immobilized to effect binding of antigens or antibodies in the sample which are specific to the immobilized component. The presence of the antigen-antibody complex may then be determined by any known means.
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