Polycos vectors
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United States of America Patent
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Dec 26, 1995
Issued Date -
N/A
app pub date -
Oct 12, 1993
filing date -
Apr 12, 1991
priority date (Note) -
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Abstract
A bacteriophage packaging site-based vector system is describe that involves cloning vectors and methods for their use in preparing multiple copy number bacteriophage libraries of cloned DNA. The vector is based on a DNA segment that comprises a nucleotide sequence defining a bacteriophage packaging site located between two termini having ligation means used to concatamerize fragments of DNA having compatible ligation means. Methods for preparing a concatameric DNA for packaging cloned DNA segments, and for packaging the concatamers to produce a library are also described.
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