Three-dimensional stromal cell and tissue culture system
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United States of America Patent
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Dec 26, 1995
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Oct 23, 1992
filing date -
Oct 23, 1992
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Abstract
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
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