Wash composition containing signal stop reagent, test kit and method of use with peroxidase-labeled specific binding ligand
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United States of America Patent
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Jan 9, 1996
Issued Date -
N/A
app pub date -
Jun 7, 1993
filing date -
Oct 8, 1991
priority date (Note) -
Expired
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Abstract
A buffered composition containing a nonionic or anionic surfactant, and a certain amount of a hydroxamic acid (or salt thereof) or an acyl hydrazine can be used as a wash composition in specific binding assays. Such assays include the use of a peroxidase labeled specific binding reactant to form a detectable peroxidase-labeled specific binding complex. The buffered composition and labeled reactant can be supplied in a diagnostic test kit. The hydroxamic acid or acyl hydrazine can be represented by the structure(I): R--CO--NH--R' wherein R is aryl or 6 to 10 carbon atoms in the aromatic nucleus, alkyl of 1 to 7 carbon atoms, cycloalkyl of 5 to 10 carbon atoms in the ring, or heterocyclyl of 5 to 10 atoms in the ring, at least one of which is an oxygen, nitrogen or sulfur atom, and R' is hydroxy or amino.
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