Immunological test for the presence of antibodies in biological fluids
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United States of America Patent
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Mar 26, 1996
Grant Date -
N/A
app pub date -
Apr 20, 1993
filing date -
Jun 20, 1991
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Abstract
Antibodies (Ak), in particular autoantibodies, such as autoantibodies against thyroid peroxidase (TPO), are determined by a procedure in which the sample to be investigated is reacted with two further, in particular monoclonal antibodies and an antigen. The two further antibodies are an immobilised antibody (Ak.sub.imm) and a freely labelled antibody (Ak*) against the antigen (Ag), and the latter is preferably used in the form of a crude, natural antigen, for example in the form of an organ extract. The presence of the antibodies (Ak) to be determined is evident as a disturbance of the formation of a sandwich of the two added antibodies (Ak.sub.imm and Ak*) with the added antigen (Ag) and as a consequent reduction in the binding of the label to the solid phase.
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