Method of detecting abnormal epitope expression on an immunocaptured constant number of protein molecules
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United States of America Patent
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May 14, 1996
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N/A
app pub date -
Jul 7, 1994
filing date -
Jun 9, 1989
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Abstract
A method is disclosed for detecting abnormal epitope expression of protein molecules by immunocapturing a constant number of protein molecules from a test and a reference sample using a limited amount of capturing antibodies, saturating them with its corresponding protein from the test and reference samples to provide a constant, known amount of the immobilized protein for comparison, labelling an epitope on the immobilized protein by means of a labelled probing antibody specifically reactive with the epitope and correlating any difference in labelling between the test and the reference sample to the absence or modification of the epitope in the protein of the test sample.
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