Leukocyte differentiation method
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United States of America Patent
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-
May 21, 1996
Issued Date -
N/A
app pub date -
Jan 28, 1987
filing date -
Sep 24, 1984
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method is disclosed for differentiating white blood cells. The method uses at least one surfactant and at least one dilute acid which together selectively strip the cytoplasm from certain classes of white blood cells and not others. More particularly, the method causes lysis of lymphocytes, monocytes, eosinophils and neutrophils, but not basophils. Thus, basophils are differentiated from other PMN sub-classes by their appearance as intact cells. The method totally avoids the need for dye preparation and the vagaries of staining techniques and can be used either manually or with instrumentation.
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