Methods, primers and probes for detection of hepatitis C and novel variants
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United States of America Patent
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Jun 18, 1996
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app pub date -
May 10, 1994
filing date -
Aug 27, 1991
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Abstract
Oligonucleotides primers can be used to amplify and detect Hepatitis C virus nucleic acids in a process that involves reverse transcription of the viral genomic RNA to create cDNA and the subsequent amplification of the cDNA by the polymerase chain reaction. Oligonucleotide probes can be used to detect the presence of amplified DNA by hybridization. The invention provides improved methods, compositions, and kits for amplifying and detecting U.S., Japan, and HCV-C prototype nucleic acids.
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